Operate the HPLC like an actual bench instrument
This simulator reproduces a full startup and routine-use workflow: solvent check, priming, column installation, method parameters, baseline equilibration, system-suitability injection, sample run and scientific review. The strongest outcome is not simply “getting peaks”; it is producing defensible analytical evidence.
Lines: Disconnected
Pressure: 0 bar
Prime: Not done
Injection: Idle
Needle wash: Ready
Temp: 25 °C
Status: Idle
Baseline: Offline
Signal: 0.0000 AU
Immediate instrument actions
Scientific note
Approved method parameters
Choose values that support the intended assay. The method is fictional, but the control logic is realistic: mobile-phase program, flow rate, wavelength, injection volume and oven temperature must be deliberately set and scientifically consistent.
Method entry panel
Expected method intent
| Parameter | Expected training value | Reason |
|---|---|---|
| Flow rate | 1.00 mL/min | Reference instrument flow for the assay |
| Wavelength | 230 nm | Training analyte absorbance maximum |
| Oven temperature | 30 °C | Controlled retention / reproducibility |
| Injection volume | 10 µL | Balances response and peak shape |
| Mobile phase | A:B 60:40 | Produces target retention / resolution in this scenario |
Chromatogram & run evidence
The traces below update after standard and sample injections. The standard injection determines whether the system is fit for use; the sample should only be interpreted if the run conditions are analytically defendable.
| Run | Retention time 1 | Retention time 2 | Resolution | Tailing | Status |
|---|---|---|---|---|---|
| Standard | — | — | — | — | Not run |
| Sample | — | — | — | — | Not run |
Recognize causes, not just symptoms
A realistic analyst does more than notice a bad chromatogram. The analyst connects the symptom to instrument state, method settings and preparatory steps to identify the most likely cause and the correct next action.
Unstable baseline
Often linked to poor equilibration, bubbles or solvent mixing inconsistency.
High pressure
Can arise from blocked frits, column issues or excessive flow / viscosity.
Poor resolution
May result from wrong mobile phase, flow rate, wrong column conditions or column deterioration.